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. 2017 May 18;8(38):62953–62961. doi: 10.18632/oncotarget.17972

Figure 2. Regulation of SAV1 expression by promoter hypermethylation in pancreatic cancer and validation of transfection.

Figure 2

(A) Diagram of CpG islands in the promoter region of SAV1. Reactivation of SAV1 expression. AsPC-1and SW1990 cells were treated with 5-azaC (5μM) for 4 and 5 days. Quantitative real-time PCR (B) and western blot (C) were used to analyze the mRNA and protein levels of SAV1, respectively. (D) Quantitative real-time PCRanalyses of SAV1 expression in PANC-1 cells underwent transient transfection of SAV1 siRNA (siRNA1, siRNA2, and siRNA3). (E) SAV1 protein expression in PANC-1 cells transfected with SAV1 siRNA (siRNA1, siRNA2, and siRNA3) was confirmed by western blot analysis. (F) PCR analyses of SAV1 expression in AsPC-1 cells underwent stably transfection. (G) SAV1 protein expression in AsPC-1 cells transfected with SAV1-constructed plasmid.