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. 2017 May 19;8(38):62962–62975. doi: 10.18632/oncotarget.18002

Figure 8. PTC-209 treatment inhibits the invasive properties of DIPG and downregulates the EMT factors ZEB1 and Vimentin.

Figure 8

(A) Experimental design of the scratch assay. (B) Representative images of the scratch assay at 0 and 48 hrs-time points post-treatment. (C) Quantifications of the scratch assay showing the percentage area covered in 48 hrs with after DMSO or PTC-209 treatment. (D) Representative immunofluorescence images GFAP (red) and Vimentin (green) expression in CCHMC-DIPG-1 and SU-DIPG-IV cells after treatment with DMSO 5 μM PTC-209 for 3 days, DAPI (blue). (E–G) ZEB1, SNAIL1 and TWIST1 expression in cells treated with DMSO or 5 μM PTC-209 for 3 days by qPCR. Error bars represent the standard deviation from three independent experiments performed in triplicates. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.