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. 2017 Jun 13;8(38):63026–63037. doi: 10.18632/oncotarget.18450

Figure 3. Effect of ZNF24 on NF-κB.

Figure 3

(A) RT-qPCR analysis of ZNF24 mRNA expression level after siRNA transfection treatment in GM0639 cells for 24 and 48 h. Nontargeting siRNA (si-NC) was used as a control. Data is represented as mean ± SD. (B) ZNF24 knockdown reduced p65 expression as determined by western blot analysis. Nontargeting siRNA treatment was used as a control. Protein loading was evaluated by β-actin. (C) Downregulation of ZNF24 markedly inhibited TNF-α-induced NF-κB activation. GM0639 cells were treated with siRNA transfection for 48 h and then with TNF-α (1 nM) for the indicated times. Nuclear extracts were prepared and analyzed by western blot using antibodies against p65 and phospho-specific p65 (Ser 536). Nontargeting siRNA treatment was used as a control. Histon-H3 was detected as the loading control. Each assay was performed in triplicate. *p < 0.001.