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. 2017 Jun 21;8(38):63038–63046. doi: 10.18632/oncotarget.18593

Figure 2. The expression level of circulating YKL-40.

Figure 2

(A) Expression of YKL-40 mRNA level was confirmed through qRT-PCR analysis in control, CHD, and DM2-CHD, respectively. Total RNA extraction was performed using TRIzol Reagent. The data were normalized to 18S rRNA in each sample. The qRT-PCR data were analyzed using the 2 −ΔΔCt method. (B) YKL-40 levels were determined using ELISA following a BCA assay for PPP (platelet poor plasma) in control, CHD, and DM2-CHD, respectively. *P<0.05, Control vs CHD, DM2-CHD; # P<0.05 vs CHD group.