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. 2017 Jun 21;8(38):63038–63046. doi: 10.18632/oncotarget.18593

Figure 3. YKL-40 is a direct target of miR-24.

Figure 3

(A) Schematic representation of the YKL-40 3′ UTR luciferase reporter plasmid. The “seed sequences” and the point mutations in the seed sequences are underlined. (B) and (C) A miR-24 mimic (50 nmol/L), miR-24 inhibitor (50 nmol/L) or control oligo (50 nmol/L) was co-transfected with the psi-CHECK-2 wild-type or mutated YKL-40 3′ UTR sequence vectors in HEK 293 cells. The relative luciferase activity is reported. All data are presented as the mean ± SEM of triplicate independent experiments.*p < 0.05, miR-24 mimic experimental vs. Blank, miR-24 inhibitor experimental, NC and NC inhibitor. #P < 0.05, miR-24 inhibitor experimental vs. Blank, miR-24 mimic experimental, NC and NC inhibitor.