Table 1. Neutralizing activity of Y498 and control mAbs on diverse clades of HIV-1 isolates.
| IC50(μg/ml)# | ||||||||
|---|---|---|---|---|---|---|---|---|
| Env clone | Origin | Accession # | Clade | Tier | Y498 | b12 | VRC01 | A16 |
| 398F1* | Tanzania | HM215312 | A | 1 | 0.19 | 0.08 | 0.08 | 1.85 |
| 92RW020.2 | Rwanda | EU855131 | A | 1 | >50 | >50 | 0.33 | >50 |
| 92UG037.8 | Uganda | U51190 | A | ? | >50 | >50 | 0.13 | >50 |
| X2278* | Spain | FJ817366 | B | 2 | >50 | >50 | 0.1 | >50 |
| TRO11* | Italy | AY835445 | B | 2 | >50 | >50 | 0.34 | >50 |
| NL4-3 | USA | AY669735 | B | 1 | 0.61 | 0.02 | 0.08 | 0.53 |
| SF162 | USA | EU123924 | B | 1 | 0.02 | 0.01 | 0.02 | 0.3 |
| JRFL | USA | AY669728 | B | 2 | >50 | 0.03 | 0.06 | 50 |
| REJO4541.67 | USA | AY835449 | B | 2 | >50 | 0.92 | 0.11 | >50 |
| RHPA4259.7 | USA | AY835447 | B | 2 | >50 | 0.1 | 0.05 | 37.5 |
| PVO.4 | Italy | AY835444 | B | 3 | 50 | >50 | 1.04 | >50 |
| pCAAN5342.A2 | USA | AY835452 | B | 2 | >50 | >50 | 1.23 | >50 |
| SC422661.8 | Trinidad | AY835441 | B | 2 | >50 | 0.2 | 0.13 | >50 |
| AC10.0 | USA | AY835446 | B | 2 | >50 | 36.92 | 2.49 | >50 |
| p1058_11.B11.1550 | USA | EU289187 | B | 2 | >50 | >50 | 31.13 | >50 |
| pWITO4160 | USA | AY835451 | B | 2 | 50 | 5.3 | 0.2 | 50 |
| ADA | USA | AY426119 | B | 2 | >50 | 0.69 | 0.14 | >50 |
| SC45.4B5.2631 | Trinidad | EU289201 | B | ? | 50 | 1.73 | 0.53 | 16.67 |
| pWEAUd15.410.5017 | USA | EU289202 | B | ? | >50 | 1.81 | 0.22 | >50 |
| B01 | China | ABY71518 | B′ | ? | >50 | >50 | 2.12 | >50 |
| B02 | China | ABY71519 | B′ | ? | >50 | 0.22 | 0.07 | 33.13 |
| B04 | China | ABY71521 | B′ | ? | 5.69 | >50 | 31.13 | >50 |
| CNE4 | China | HM215413 | B | ? | >50 | >50 | 0.83 | 11.02 |
| CNE11 | China | HM215398 | B′ | ? | >50 | 1.58 | 0.23 | >50 |
| CNE14 | China | HM215400 | B′ | ? | >50 | 2.21 | 0.56 | >50 |
| 25710* | India | EF117271 | C | 1 | 50 | >50 | 0.36 | >50 |
| CE0217* | Malawi | FJ443575 | C | 3 | >50 | 18.22 | 0.32 | >50 |
| CE1176* | Malawi | FJ444437 | C | 2 | >50 | >50 | 1.83 | >50 |
| DU422.1 | South Africa | DQ411854 | C | 2 | 50 | 0.2 | >50 | >50 |
| ZM214M.PL15 | Zambia | DQ388516 | C | 2 | >50 | >50 | 2.64 | >50 |
| CAP210.2.00.E8 | South Africa | DQ435683 | C | 2 | 50 | >50 | >50 | >50 |
| CAP45.2.00.E8 | Durban | DQ435682 | C | 2 | >50 | 2.3 | 8.95 | >50 |
| ZM109F.PB4 | Zambia | AY424138 | C | 1 | >50 | >50 | 0.11 | >50 |
| CNE2 | China | HQ699950.1 | C | ? | 9.08 | 0.45 | 0.05 | >50 |
| CNE17 | China | HM215303 | C | ? | 14.22 | >50 | 1.27 | 42.86 |
| CNE58 | China | HM215421.1 | C | ? | >50 | >50 | 0.14 | 50 |
| CNE65 | China | HQ699980.1 | C | ? | >50 | >50 | 0.96 | >50 |
| 246F3* | Tanzania | HM215279 | A/C | 2 | >50 | >50 | 0.41 | >50 |
| CNE3 | China | HM215410 | A/E | ? | >50 | >50 | 4.94 | >50 |
| CNE8* | China | HM215427 | A/E | 2 | >50 | >50 | 0.43 | >50 |
| CNE55* | China | HM215418 | A/E | 3 | >50 | >50 | 0.42 | >50 |
| CNE59 | China | HM215422.1 | A/E | ? | >50 | >50 | 34.15 | 50 |
| YN192.31 | China | GU475046 | A/E | ? | >50 | >50 | 0.32 | >50 |
| GX2010.36 | China | ADD83183 | A/E | ? | 50 | >50 | 3.22 | 26.93 |
| AE03 | China | EU363851.1 | A/E | ? | >50 | >50 | 0.01 | >50 |
| BJOX2000* | China | HM215364 | B/C | 2 | >50 | >50 | >50 | >50 |
| CH119* | China | EF117261 | B/C | 2 | 36.47 | >50 | 0.97 | 5.56 |
| XJ16-6 | China | HQ326133.1 | B/C | ? | >50 | 10.75 | 3.5 | >50 |
| HB5-3 | China | ADT65143 | B/C | 2 | >50 | >50 | 0.27 | >50 |
| SC19-15 | China | ADT65148 | B/C | ? | >50 | 26.59 | 0.19 | 50 |
| YN148r-9 | China | ADT65158 | B/C | ? | >50 | 1.27 | 2.02 | >50 |
| BC07 | China | ABY71530 | B/C | ? | >50 | >50 | 0.18 | >50 |
| CNE7 | China | HM215426 | B/C | ? | >50 | 2.25 | 0.56 | >50 |
| CNE15 | China | HM215401 | B/C | ? | >50 | 12.75 | 0.07 | >50 |
| CNE16 | China | HM215402 | B/C | ? | >50 | 9.62 | 0.14 | >50 |
| CNE23 | China | HM215408 | B/C | ? | >50 | 5.06 | 16.29 | >50 |
| CNE30 | China | HM215411 | B/C | ? | 50 | 21.83 | 0.59 | >50 |
| CNE40 | China | HM215414 | B/C | ? | 0.07 | >50 | 0.3 | 0.26 |
| CNE46 | China | HQ699971 | B/C | ? | >50 | 24.63 | 3.03 | >50 |
| CNE47 | China | HQ699972 | B/C | ? | >50 | >50 | 29.02 | >50 |
| CNE49 | China | HQ699974 | B/C | ? | >50 | >50 | 0.31 | >50 |
| CNE53 | China | HM215417 | B/C | ? | 32.73 | >50 | 0.11 | 14.51 |
| CH064.20 | China | EF117254 | B/C | 2 | >50 | 19.28 | 0.55 | 50 |
| CH070.1 | China | EF117255 | B/C | 3 | >50 | >50 | 10.76 | 50 |
| CH091.9 | China | EF117256 | B/C | 2 | 6.7 | >50 | 0.23 | 5.83 |
| CH110.2 | China | EF117257 | B/C | ? | >50 | 10.8 | 4.16 | >50 |
| CH114.8 | China | EF117264 | B/C | 3 | 31.6 | >50 | 0.29 | 45.3 |
| CH117.4 | China | EF117262 | B/C | 2 | 50 | >50 | 0.11 | 50 |
| CH120.6 | China | EF117260 | B/C | 3 | 50 | >50 | 4.5 | 50 |
| X1632* | Spain | FJ817370 | G | 2 | >50 | >50 | 0.29 | >50 |
| % of neutralization | 30% | 42.86% | 95.71% | 32.86% | ||||
* represents a ‘global panel’ HIV-1 isolates (reference 29); ? means ‘not determined’;
# indicates that the IC50 values of three control mAbs were obtained from a previous study (reference 13). The neutralizing IC50 values are colored to show the strength (10-50 = green; 1-10 = light green; 0.1-1= yellow; .01-.1= red).