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. 2017 Jun 27;8(38):63110–63120. doi: 10.18632/oncotarget.18668

Figure 2. shKMT2A induces U-87 MG cell proliferation.

Figure 2

(A) The effect of shKMT2A was examined using a WST-1 assay, which demonstrated that shKMT2A induces U-87 MG cell proliferation. (B) Cell proliferation and survival markers were analyzed using real-time RT-PCR. shKMT2A induces proliferation markers [Ki-67, PCNA, Topoisomerase IIα (TopoIIα), and TPX2] without a significant alteration of apoptosis markers (BAX, P38 MAPK, and P53). (C) Cell growth assay showing shKMT2A induces U-87 MG cell growth. (D) Western blot analysis indicated that shKMT2A upregulates the expression level of PCNA. (E) Representative images revealing that shKMT2A induces the growth of U-87 MG cells. Cells were stained with crystal violet. *, p < 0.05; **, p < 0.01; ***, p < 0.001.