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(A) KMT2A knockdown-induced cell proliferation was recovered using N1ICD or N3ICD, as determined by the WST-1 assay, cell growth assay, and Western blot analysis with PCNA antibody in U-87 MG cells. (B) shNOTCH1 or shNOTCH3 in shKMT2A-transfected U-87 MG cells did not further induce U-87 MG cell proliferation, compared with the effect of shKMT2A. n.s., not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001.
