Table 4. MSP primer sequences.
Name | Lightcycler MSP primers | |
---|---|---|
S primer | AS primer | |
BIK | TTTTTGGAGTTTCGGTTTTTAC | CTTTACACGAATAACCTCCGTTC |
CAT | GTTTGTTGTTTCGAGTTCGTG | ATCTTAACCTACCTAACGCCGA |
CCND2 | CGGGGTTGTTTTATTCGTATCG | CAACCAACTTACGTCACCGCT |
CDK5 | AGTTTTGCGGGAAATGTTAATAC | AAACTCCGATCTCAACAACGA |
DAPK1 | TAAGGAGTCGAGAGGTTGTTTC | CCTACCGCTACGAATTACCGA |
DCR1 | TTACGCGTACGAATTTAGTTAAC | CATCAAACGACCGAAACG |
EEF1A2 (1) | GTTCGTGATTAGTAGAGTCGGGT | ACAACGAATAAAAATAAAACGCC |
EEF1A2 (2) | TTAGGTTGGGTACGTTCGTGA | ACAACGAATAAAAATAAAACGCC |
HOXA9 | AGGAGCGTATGTATTTGTCGTTC | AACGCTATACCCGCTACGATA |
IRAK1 | AGGATGTGTACGAGGTCGGTT | CGAACTACGACTATACGAACGCT |
LIG4 | GAGTTAAAAACGGGAGAAATCGT | CACAACGCTATAAACTACGCC |
NUDT1 | GTATTTTTCGAGTTCGTTACGTTT | TCCTCTTAACGTCCAACGAC |
PAX3 (1) | TTTGGGTATAGCGTCGGTT | ATTCCCGAAAATCATCCGC |
PAX3 (2) | ATAGTTTTCGAGGGTTATTCGC | CCTAAACACAACGCCGACC |
PRKCB1 | GTATCGCGTTTAGGTTTCGTTT | CCGACGCTACAAAACTACGA |
PROK2 | ATAAAGGTTAGTTTCGTCGTGA | ACACGTACTCGTCTAAAAACCG |
PROP1 | CGAGTTATGGAAGTAGAAAGGAGGC | ATAATCGAAATCCCAATAACCGA |
PTGS2 | TTACGGAAATGAGAAAATCGG | GCCTAAAACGATAAAACTCGAAA |
RASSF1 | GCGTATTGTAGGTTTTTGCGT | TAATCCCTAACCGTAACCACCG |
RBBP8 | GTATTTTTATACGGGTAAGGCGA | TACCCCGCTACTCTACTCCGC |
RHOB | AGGAGGGGATTCGGGTATC | TAATTAACGACCCAAACCG |
SPO11 | AGTGTGGGTCGCGTAGGTATC | CTAAATCCAATATCCGCAACACG |
TBX5 | TCGGTATTGATAGGCGAAGAC | CTATAAAACTTAAAAACGTCACGAA |
TIPARP | TAAGGTTTACGAAATAGTCGGTC | ACTACCACCAAACGAAATCGC |
Beta Actin | TAGGGAGTATATAGGTTGGGGAAGTT | AACACACAATAACAAACACAAATTCAC |
Name | qMSP primers | |
S primer | AS primer | |
Beta Actin | TGGTGATGGAGGAGGTTTAGTAAGT | GAATTTTTTTATGTGTATGAATTTAGTTAAT |
DCR1_U | GAATTTTTTTATGTGTATGAATTTAGTTAAT | CCATCAAACAACCAAAACA |
DCR1_M | TTACGCGTACGAATTTAGTTAAC | CATCAAACGACCGAAACG |