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. 2017 Jun 28;8(38):63265–63280. doi: 10.18632/oncotarget.18792

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Copyright: © 2017 Aharon et al.

This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.

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Human umbilical vein endothelial cells (HUVEC) were seeded for 20 hours with or without EV pellets (25μg) obtained from healthy controls (HC) and BC patients. (a) EV effect on EC thrombogenicity measured by FXa chromogenic assay. Results are expressed as TF arbitrary units. (b) EV effects on EC apoptosis measured by TUNEL assay. (c) EV effects on EC proliferation measured by the XTT assay. (d) EV effects on EC migration validated using the Boyden chamber. The area occupied by migratory cells was photographed by light microscopy (x10 magnification) (d1) and calculated using image J software (d2).