Figure 2. HMGB1 does not influence the development and proliferation of T cells and B cells.

Bone marrow cells were stimulated with HMGB1 (10 μg/mL), αHMGB1Ab (2 μg/mL), αHMGB1Ab (10 μg/mL), PBS (10 μg/mL) or isotype control Ab (10 μg/mL) in vitro. (A, B and C) Two days later, the percentage and cell number of T and B cells were detected by FACS and trypan blue assay. Data are mean±SEM. Representative results of one of three independent experiments.