Figure 2. The Anapc2 deletion resulted in BM failure.

(A) Survival curve of Anapc2 cKO and Anapc2^flox/flox mice (Ctrl) after the pIpC injection (n=30). Most Anapc2 cKO mice died within 40 days. (B and C) Peripheral white blood cell (WBC) and hemoglobin (Hb) counts of Anapc2 cKO mice significantly decreased at day 5 after the pIpC injection. ****p<0.0001. (D) HE staining analysis of BM sections from the femur of Anapc2 cKO mice and control (Ctrl). After the pIpC injection, most nucleated cells disappeared in the BM. Scale bar represents 100 μm. (E) HE staining analysis of BM sections from the femur of Anapc2 cKO mice and control (Ctrl). An increase of mitotic cells which were arrested in a metaphase-like state (green arrow) was observed in the bone marrow before the disappearance of hematopoietic cells. Scale bar represents 100 μm.