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(A-C) Anapc2 could not be detected in the BM CD34+ cells of AA by bi-color IHC staining. In normal control (Ctrl), Anapc2 (expressed in nuclear, golden in color) could be detected in the BM CD34+ (expressed in membrane, blue in color) cells by bi-color IHC staining (green arrow) whereas it could not in AA's (green arrow). n=3 (Ctrl), 20 (AA), scale bar represents 100 μm, ****p<0.0001.
