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. 2017 Jun 29;8(38):63646–63664. doi: 10.18632/oncotarget.18882

Figure 8. Transcriptional up-regulation of XIAP by TGF-β/Smad signaling.

Figure 8

(A) Real-time RT-PCR analysis (left) of XIAP mRNA expression, and western blot analysis (right) of indicated proteins for the times shown after 2 ng/mL TGF-β treatment of Ishikawa cell lines. (B) Ishikawa cells were transfected with XIAP reporter constructs, together with Smad2 and LEFTY1. Relative activity was determined by arbitrary light units of luciferase activity normalized to pRL-TK activity. The activities of the reporter plus the effector relative to that of the reporter plus empty vector are shown as mean±SD. The experiment was performed in duplicate. (C) Conventional RT-PCR analysis of endogenous XIAP mRNA expression in TOV-L1 and ES-L1 stable cells. (D) Staining was by HE and IHC for LEFTY, pSmad2, and XIAP in semi-serial sections of OCCCa. Original magnification, x200. (E) Correlations of XIAP score with pSmad2 (upper) and LEFTY scores (lower) in OCCCa.