Figure 6. miR-223 overexpression recapitulated EMT induction in LoVo cells.

LoVo cells were transiently transfected with miR-223 mimics, miR-223 inhibitor, or negative control, respectively, and were transfected with p120 siRNA, p120 1A or p120 3A plasmid as control. 48 h after transfection, p120, E-cad, and VIM were detected via western blotting, with actin as the loading control (A). Representative images shown are from independent experiments repeated three times. Quantified western blotting results (B). n=3; *P<0.05 vs. the control group.