Figure 9. miR-223 overexpression enhances RhoA activity in LoVo cells.

LoVo cells were transiently transfected with miR-223 mimics, miR-223 inhibitor, or negative control, respectively, and were transfected with p120 siRNA, p120 1A, or p120 3A plasmid as control. 48 h after transfection, RhoA and Rac1 were detected by western blot, with actin as the loading control (A). Representative images shown are from independent experiments repeated three times. Quantified western blotting results (B). LoVo cells were transiently transfected with miR-223 mimics, miR-223 inhibitor, negative control, or p120 siRNA. The activity of RhoA was detected by G-LISA (C). n=3; *P<0.05 vs. the control group.