Table 1. Names and sources of antibodies used in flow cytometry and Western blot.
Name of antibody (usage) | Catalog number | Company |
---|---|---|
Mouse anti-human TACE (FCM) | ab2051 | Abcam |
APC anti-human TNFR1 (FCM) | 369905 | Biolegend |
PE anti-human TNFR2 (FCM) | 358403 | Biolegend |
PE anti-human DR5 (FCM) | 307405 | Biolegend |
PE anti-human DR4 (FCM) | 307205 | Biolegend |
Mouse ani-human CD3 mAb (restimulation) | 300314 | Biolegend |
APC anti-human TRAIL(FCM) | sc-56246 | Biolegend |
Rabbit anti-human β-actin pAb (WB) | AC026 | ABclonal |
FITC-conjugated goat anti-mouse IgG (FCM) | 31561 | Thermo Fisher scientific |
HRP-conjugated goat anti-rabbit IgG (WB) | 62-9520 | Thermo Fisher scientific |
Rabbit anti-human TNF-α pAb (stimulation or WB) | / | House Made |
mouse anti human TNF-α mAb (FCM) | / | House Made |
FITC anti-human Fas (FCM) | 11-0959 | eBioscience |
PE anti-human FasL (FCM) | 12-9919 | eBioscience |
FCM: Flow cytometry; WB: Western blot.