Table 3. Detailed aspects for each antibody used in immunohistochemistry.

Protein	Antigen retrieval	Antibody (product # and company)	Antibody dilution andincubation time
MCT1	Citrate buffer (0.01 M, pH=6), 98°C, 20′	AB3538P Merck-Millipore	1:200, overnight
MCT2	Citrate buffer (0.01 M, pH=6), 98°C, 20	sc-50322 Santa Cruz Biotechnology	1:200, 2 hours
MCT4	Citrate buffer (0.01 M, pH=6), 98°C, 20′	sc-50329 Santa Cruz Biotechnology	1:500, 2 hours
CD147	EDTA (1 mM, pH=8), 98°C, 20′	sc-71038 Santa Cruz Biotechnology	1:400, overnight
CD44	Citrate buffer (0.01 M, pH=6), 98°C, 20	MCA2726 AbD Serotec	1:2000, 2 hours
GLUT1	Citrate buffer (0.01 M, pH=6), 98°C, 20′	ab15309-500 AbCam	1:500, 2 hours
CAIX	Citrate buffer (0.01 M, pH=6), 98°C, 20′	ab15086 AbCam	1:2000, 2 hours