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. 2017 Jul 12;8(38):63935–63948. doi: 10.18632/oncotarget.19212

Figure 5. Knockdown of HOXA9 suppresses CRC tumorigenesis and metastasis.

Figure 5

HCT8 cells were infected with a lentivirus expressing specific shRNA of HOXA9 genes (HOXA9-KD) or shRNA scramble controls (Lv-scramble). (A) qPCR analysis of the knockdown effect in HCT8 and HCT116 cells infected with HOXA9-KD or Lv-scramble. (B) in vitro cell proliferation was detected with CCK8 assay. (C) in vitro cell proliferation was detected with colony formation assay. (D, E) In vitro cell migration was analyzed with a gel invasion assay (D) and a wound-healing assay (E). (F) Knock down of HOXA9 decreased ZEB1 while increased E-cadherin expression. (G) Overexpression of miR133-b decreased HOXA9 and ZEB1, while increased E-cadherin expression. *P < 0.05, versus scramble control.