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. 2017 Jul 12;8(38):63949–63962. doi: 10.18632/oncotarget.19213

Figure 2. Isothiocyanates inhibit the activity of AP-1 and NF-κB in the MMP-9 promoter in U2OS cells.

Figure 2

(A) U2OS cells were treated with TPA and/or isothiocyanates for 24 h. Expression of mRNA encoding MMP-9 and TIMP-1 was then analyzed by RT-PCR. (B) U2OS cells were treated with TPA and isothiocyanates for 24 h, and the expression of p65, c-Fos, and c-Jun, and Lamin B (internal control) was analyzed by western blotting following nuclear extraction. (C–E) U2OS cells were transfected with the pGL2-MMP-9-WT, pGL2-MMP-9-mAP-1, and pGL2-MMP-9-mNF-κB reporter plasmids and then treated with TPA and isothiocyanates for 24 h. Relative luciferase activity in the cell extract was then measured.