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. 2017 Jul 15;8(38):63986–64000. doi: 10.18632/oncotarget.19256

Figure 5. REGγ is a direct downstream target of miR-195-5p in RCC.

Figure 5

(A) REGγ was predicted as a direct target of miR-195-5p by bioinformatic analysis using the TargetScan, PicTar and miRanda databases. The overlap represents the same prediction derived by 3 databases. (B) The sequences of miR-195-5p binding sites in the 3′-UTR of wild-type (wt) REGγ mRNA and of a mutant (mut) 3′-UTR of REGγ. (C) The relative luciferase activity of the wt REGγ 3′-UTR and mut REGγ 3′-UTR reporters in 786-O cells in the presence of miR-195-5p mimics (miR-195-5p) or the negative control (miR-NC). (D and E) The relative REGγ mRNA levels in 786-O and caki-1 cells as determined by qRT-PCR following transfection with miR-195-5p mimics (D) or inhibitors (E). 18S was used as an internal control. (F and G) Western blot measurement of REGγ protein expression in 786-O and caki-1 cells following transfection with miR-195-5p mimics (F) or inhibitors (G). (H) The expression of REGγ was evaluated by immunohistochemistry in tumor tissues derived from a xenograft nude mice model. Scale bar= 100 μm. The data are presented as mean ± SD of 3 independent experiments. *P < 0.05