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. 2017 Jul 17;8(38):64050–64065. doi: 10.18632/oncotarget.19300

Figure 5. The effect of the interaction between Sp110 and Hspa5 on Sp110 enhances macrophage apoptosis.

Figure 5

(A) Schematic illustration of RAW264.7 cells expressing full-length Sp110 or mutants (top panel). Lysates from RAW264.7 cells expressing the Sp110 mutants were subjected to IP (bottom panel). (B) RAW264.7 cells were cotransfected with full-length Sp110 or mutants plus pCMV-HA-Hspa5 for 24 h, and then the subcellular localizations of Sp110 and Hspa5 were examined by immunofluorescence staining. Nuclei were stained with DAPI. Scale bar = 25 μm. (C) RAW264.7 cells expressing full-length Sp110 or mutants were infected with H37Ra (MOI 5:1) for 24h. Cell apoptosis was quantified by Annexin-V staining followed by flow cytometric analysis.