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. 2017 Jul 18;8(38):64114–64128. doi: 10.18632/oncotarget.19342

Figure 2. DKK3 is a functional target of miR-708 in B-ALL.

Figure 2

(A) Predicted binding sites for miR-708 and DKK3. The sequence of the mutant 3′-UTR of DKK3 is also presented. (B) Luciferase assays showing the decrease in relative luciferase activity in Nalm-6 cells co-transfected with miR-708 and DKK3 and the increase in cells co-transfected with a miR-708 inhibitor and DKK3. A mutated 3′-UTR DKK3 plasmid was used as the control. (C-H). The miR-708 (C and F) and DKK3 mRNA levels (D and G) and the DKK3 protein expression levels (E and H) changed after transfection with a miR-708 mimics or a miR-708 inhibitor, respectively. (D and G). The graphs show the fold changes in protein expression relative to the untreated control measured by densitometry. (E and H) The images represent the results of a western blotting analysis of DKK3 protein expression. The data are presented as the means ± SDs of three different experiments. NC indicates the negative control. *P<0.05; **P<0.01.