Table 2. Methylation levels of TBX21, GATA3, RORγt, FOXP3, IFN-γ, IL-4, IL-17A and TGF-β promoter in CD4+ T cell from BD patients versus normal controls.
| Gene | CG sites | BD methylation level (%,mean ± SD) | CN methylation level (%,mean ± SD) | p value |
|---|---|---|---|---|
| CG2 | 63.8±3.6 | 62.5±7.7 | 0.53 | |
| TBX21 | CG3 | 7.3±1.1 | 7.9±1.7 | 0.30 |
| CG4 | 8.8±1.6 | 9.4±2.0 | 0.61 | |
| CG1 | 2.8±1.8 | 3.1±2.1 | 0.80 | |
| CG3.4 | 6.5±1.6 | 5.8±1.9 | 0.43 | |
| GATA3 | CG5 | 3.9±0.01 | 3.2±0.02 | 0.33 |
| CG6 | 4.7±3.3 | 2.9±1.9 | 0.13 | |
| CG7.8.9 | 7.2±1.9 | 5.3±1.2 | 0.001** | |
| RORγt | CG5 | 67.1±7.1 | 69.5±6.7 | 0.34 |
| CG2 | 36.7±14.3 | 34.1±10.1 | 0.30 | |
| FOXP3 | CG3 | 38.6±15.9 | 35.8±6.3 | 0.86 |
| CG6 | 70.5±11.7 | 68.1±13.7 | 0.23 | |
| IFN-γ | CG3 | 68.1±11.6 | 66.2±10.8 | 0.64 |
| IL-4 | CG2 | 75.4±9.3 | 65.9±10.9 | 0.012* |
| CG5 | 81.0±6.3 | 78.3±6.5 | 0.22 | |
| IL-17A | CG1 | 82.3±10.8 | 82.2±4.7 | 0.98 |
| CG2.3.4.5 | 7.8±3.2 | 4.5±1.5 | 4.65×10−4*** | |
| TGF-β | CG6 | 4.5±5.2 | 2.9±2.4 | 0.70 |
| CG10.11 | 10.0±3.1 | 6.5±1.7 | 2.85×10−4*** | |
| CG13.14 | 2.3±2.2 | 1.9±1.7 | 0.65 |
BD patients (n=16, M: F=14:2); Age (year: mean±SD): 34.63 ± 6.98.
Normal controls (n=18, M: F=14:4); Age (year: mean±SD): 37.61± 13.63.
* P < 0.05, ** P < 0.01, *** P < 0.001.