Figure 3. Dorsal-ventral HF interactions produce bilateral symmetry.
(A) Time-lapse bioluminescence in dorsal and ventral skin of the representative Flash mouse between days P5-P48. Bioluminescent signal is color-coded according to the colorimetric scale on the right. (B) Combined temporal dynamics (from 6 Flash mice) of the bioluminescent signal-based anagen measurements over four hair cycles (days P5-P119). Dorsal skin dynamics are in brown and ventral skin dynamics are in blue. Prominent temporal advancement of ventral over dorsal anagen initiation can be seen during second, third and fourth cycles (light blue areas). This advancement is accompanied by dominant ventral-to-dorsal anagen wave spreading. (C, C’) Mapping of Flash-based anagen reveals ventral-to-dorsal hair growth wave propagation and bilateral pattern symmetry. New anagen areas for each time point are color-coded. Second anagen initiation is shown on panel C, and third anagen initiation on panel C’. Also see Appendix 1—figure 6. (D–G) Hair growth distribution patterns on P17 (D), P21 (E), P39 (F) and P55 (G). Three mice were analyzed at each time point. Inverted whole mount skin samples from representative mice are shown. Schematic pattern maps are provided with color-coded anagen (green), catagen (yellow) and telogen (red) regions. Also see Appendix 1—figures 7–12. (H, I) HF cycling dynamics in chin skin grafts remain faster compared to dorsal skin grafts. After transplantation, first anagen initiated similarly in both chin and dorsal skin grafts, however, second anagen initiated significantly faster in chin grafts. Representative chin and dorsal skin grafts are shown on (H). Combined temporal dynamics of skin grafts in anagen and telogen are shown on (I). Dorsal graft dynamics are in brown and chin graft dynamics are in blue. Temporal advancement of chin over dorsal second anagen initiation is highlighted with light blue color. Also see Appendix 1—figure 13.