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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Biomaterials. 2017 Aug 15;145:44–55. doi: 10.1016/j.biomaterials.2017.08.025

Figure 5.

Figure 5

Fluorescence staining of actin (green) and nuclei (blue) of hMSCs in the GSSR5 composites in growth medium at day 19 (A). HMSCs spread out actin filaments, aligned with the pores and evenly distributed in the GSSR5 composites after 19-day culture. Live/dead staining of hMSCs cultured in the GSSR5 composites in growth medium at day 3 (B), day 11 (C) and day 19 (D). Strong green fluorescence with minimal visible red fluorescence demonstrated the cytocompatibility of the material. Alamar blue assay (E) showed the proliferation of hMSCs in the gradient (square), uniform (circle) and unsilicified plain silk control (triangular) composites over a 19-day period (n>3). Uniform silicified silk/R5 composites had a silk/R5 molar ratio of 1/125. Unsilicified plain silk composites served as controls. Scale bar, 200 μm. (*Significantly different from day 3 (p < 0.01))