Supplementary Figure S3.

Glial (GFAP and CNPase), neuronal (NeuN), and proliferating (Ki-67) cells in glioma tissues and cultured glioma cells. (A) Diversity of tumors was evident from paraffin sections of patient gliomas in which hematoxylin (blue color) was used as counterstain. Some tumors, e.g., oligodendroglioma 004-OD, were morphologically compact. H&E staining shows gemistocytes with large eosinophilic cytoplasm and peripheral nuclei in 015-OA parental tumor. Notable are, for example, the highly proliferating, Ki-67–positive (brown color) cells in glioblastomas and anaplastic oligodendroglioma. 014-OAD was used as control in all cases except in NeuN staining in which neurons from rat brain are shown. Scale bar 100 μm. (B) Fluorescence images of immunocytochemically (red color) stained cultured cells. GFAP images do not contain nuclei stain. In the other images, nuclei are stained with DAPI (blue color). Cultured cells derived from patient gliomas possessed astrocytic marker GFAP and, to a lesser extent, oligodendrocytic marker CNPase. A172 cells and primary cultures from rat cortex were used as positive controls. Scale bar 50 μm and 20 μm in GFAP and other images, respectively.