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. 2017 Sep 19;47(3):552–565.e4. doi: 10.1016/j.immuni.2017.08.003

Figure 3.

Figure 3

Mm PGL Recruits Monocytes through STING-Dependent ccl2 Induction

(A) ccl2 messenger RNA levels (mean ± SEM of three biological replicates) induced at 3 hr after caudal vein infection of 2 dpf wild-type or Sting-deficient fish with 250–300 wild-type Mm. Student’s unpaired t test.

(B and C) In situ hybridizations against zebrafish ccl2 mRNA following hindbrain ventricle infections with 80 wild-type Mm into wild-type (B) or Sting-deficient (C) zebrafish. Black arrows, ccl2 mRNA-positive phagocytes; white arrows ccl2 mRNA-negative phagocytes. Scale bar, 50μm. Results representative of three independent experiments.

(D) Mean resident macrophage and monocyte recruitment from 5 to 180 mpi in the HBV of wild-type or Sting-deficient fish after infection with 80 wild-type Mm.

(E) Mean resident macrophage and monocyte recruitment from 5 to 180 mpi in the HBV of wild-type or Sting-deficient fish after infection with 80 Mm - PDIM-.

(F) Percentage of infected (black) or uninfected (gray) wild-type or Sting-deficient fish 5 dpi with 1-3 wild-type Mm into the HBV. n = number of larvae per group. Results representative of two independent experiments. Significance testing done using Fisher’s exact test.

(G) ccl2, ifnΦ1, ifnΦ2, and ifnΦ3 mRNA levels (mean ± SEM of three biological replicates) induced at 3 hr after caudal vein infection of 2 dpf wild-type fish with 250–300 wild-type Mm. Significance testing done using Student’s unpaired t test for each gene. p = 0.002 for ccl2, all other comparisons not significant.

(H) Mean resident macrophage and monocyte recruitment from 5 to 150 mpi in the HBV of wild-type fish after infection with 80 wild-type or ESX-1-deficient (ESX1−) Mm.

(I) Percentage of infected (black) or uninfected (gray) wild-type fish 5 dpi of 1–3 wild-type, ESX1−, or PGL− Mm into the HBV. n = number of larvae per group. Significance testing done using Fisher’s exact test for comparisons shown. ∗∗p < 0.01, ∗∗∗p < 0.001. Results representative of two independent experiments.

Results in (D), (E), and (H) representative of three independent experiments.