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. 2016 Nov 17;174(20):3425–3442. doi: 10.1111/bph.13650

Figure 3.

Figure 3

Role of PVAT in obesity‐induced vascular dysfunction. C57BL/6J mice were fed a HFD or normal control diet (NCD) for 20 weeks starting at the age of 8 weeks. The vasodilator response to acetylcholine (A–C) was performed in noradrenaline‐precontracted aorta with or without PVAT in the absence or presence of the NO synthase inhibitor L‐NAME. ***P < 0.001, n = 8. To detect PVAT NO production, NCD and HFD aorta samples were mounted back‐to‐back on the same slide to guarantee identical staining conditions for the two samples (D). NO production in PVAT‐containing aorta was determined by 4,5‐diaminofluorescein diacetate (DAF‐2 DA) staining. From Xia et al. (2016) with permission of Wolters Kluwer Health, Inc. Copyright © 2016, Wolters Kluwer Health.