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. 2017 Jan 31;174(20):3443–3453. doi: 10.1111/bph.13703

Figure 6.

Figure 6

WS® 1442 normalizes eNOS acetylation level in PVAT. Male C57BL/6J mice were put on a NFD or HFD for 22 weeks starting at the age of 8 weeks. A subgroup of HFD animals were treated with WS® 1442, p.o., during the last 4 weeks of HFD feeding. Immunoprecipitation (IP) was performed with an anti‐eNOS antibody using PVAT samples followed by immunoblotting (IB) with antibodies against eNOS or acetyl‐lysine respectively. The band detected with the antibody against acetyl‐lysine at the level of eNOS was considered acetyl‐eNOS. In (A) the blots shown are representative of six independent experiments. (B) Results of densitometric analyses for acetyl‐eNOS normalized to total eNOS; n = 6. (C,D and E) Expression of SIRT1 and NAMPT at the protein (C and D) or mRNA (E) levels was analysed by western blotting and qPCR respectively. In (C) the blots shown are representative of six independent experiments. (D and E) Results of densitometric analyses are shown; n = 6. Columns represent mean ± SEM. *P < 0.05.