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. 2017 Sep 19;47(3):466–480.e5. doi: 10.1016/j.immuni.2017.08.005
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

PE anti-mouse CD34 Antibody, Clone HM34 BioLegend Cat#152204; RRID: AB_313659
PE/Cy7 anti-mouse Ly-6G Antibody, Clone 1A8 BioLegend Cat#127618; RRID: AB_1877261
APC/Cy7 anti-mouse CD117 (c-kit) Antibody, Clone 2B8 BioLegend Cat#105826; RRID: AB_2632809
APC anti-mouse TER-119/Erythroid Cells Antibody, Clone Ter119 BioLegend Cat#116212; RRID: AB_313713
PE anti-mouse CD11b Antibody, Clone M1/70 BioLegend Cat#101208; RRID: AB_312790
APC anti-mouse/human CD45R/B220 Antibody BioLegend Cat#103212; RRID: AB_312996
APC anti-mouse CD8a Antibody, Clone 53-6.7 BioLegend Cat#100712; RRID: AB_312751
APC anti-mouse CD19 Antibody, Clone 6D5 BioLegend Cat#115511; RRID: AB_313646
Anti-mouse monoclonal G-CSF R/CD114 Antibody (723806); Alexa Fluor-405 R&D Systems Cat#MAB6039; RRID: AB_10890232
APC anti-mouse CD4 Antibody, Clone GK1.5 BioLegend Cat#100412; RRID: AB_312696
Alexa Fluor 488 anti-mouse CD36 Antibody, Clone G8.8 BioLegend Cat#102607; RRID: AB_1134107

Bacterial and Virus Strains

Staphylococcus aureus ATCC ATCC 29213

Chemicals, Peptides, and Recombinant Proteins

May Grünwald Wright Giemsa stain Thermo Scientific Cat#69027
Glut1.RBD reagent GFP labeled Metaflora Cat#Glut1-G25
Recombinant murine IL-3 Peprotech Cat#213-13-100
Recombinant murine G-CSF Peprotech Cat#250-05-100
Lysostaphin from Staphylococcus Sigma Aldrich Cat#L7386
Etomoxir sodium salt hydrate Sigma Aldrich Cat#E1905
Diethyl-umbelliferyl phosphate Sigma Aldrich Cat#D7692
Orlistat Sigma Aldrich Cat#O4139
TaqMan Gene Expression Master Mix Life Technologies Cat#4369016

Critical Commercial Assays

ATP BIOLUMINESCENCE ASSAY KIT HS II Sigma Aldrich Cat# 11699709001
Anti-Ly-6G MicroBead Kit, mouse Miltenyi Biotec Cat#130-092-332
SuperScript III Platinum One-Step qRT-PCR Kit Thermo Fisher Cat#11732020
Glycolysis Cell-Based Assay Kit Cambridge Bioscience Cat#600450
Seahorse CF Cell Mito and Glycolysis Stress Test Kits Agilent Cat#103015
GLUCOSE (GO) Assay kit Sigma Aldrich Cat#GAGO20

Experimental Models: Cell Lines

32Dcl3 Cell line ATCC ATCC; CRL-11346
RRID; CVCL_0119

Experimental Models: Organisms/Strains

Mouse; Atg7 flox, Atg7tm1Tchi; B6.Cg-Atg7 < tm1Tchi > Komatsu et al., 2005 MGI: 3587769
Mouse; Atg5 flox, B6.129S-Atg5 < tm1Myok > Riken RBRC: 02975
Mouse; Mx1-cre; C.Cg-Tg(Mx1-cre)1Cgn/J Jackson Laboratories JAX: #005673
Mouse; Vav-cre; B6.Cg-Tg(Vav1-icre)A2Kio/J Jackson Laboratories JAX: #008610
Mouse: Cebpa-cre; Cebpatm1.1(cre)Touw Wölfler et al., 2010 MGI: 4867437

Oligonucleotides

Mx1Cre 1 5′ -CAT GTG TCT TGG TGG GCT GAG-3′ Sigma Oligo N/A
Mx1Cre 2 5′ -CGC ATA ACC AGT GAA ACA GCA T-3′ Sigma Oligo N/A
CebpaCre FW: 5′ -CGA TGC AAC GAG TGA TGA GGT TC-3′ Sigma Oligo N/A
CebpaCre RV: 5′ -GCA CGT TCA CCG GCA TCA AC-3′ Sigma Oligo N/A
Atg7 WT PCR 5′ -CCA TGC TGA TGG CTA ATG TCT C- 3′ Sigma Oligo N/A
Atg7 flox FW 5′ -CTG CAG GAA TTC GAT ATC ATA ACT TCG- 3′ Sigma Oligo N/A
Atg7 flox RV 5′ -GTC CAG AGT CCG GTC TCT GGT TG- 3′ Sigma Oligo N/A
Atg5 flox Primer A 5′-GAA TAT GAA GGC ACA CCC CTG AAA TG-3′ Sigma Oligo N/A
Atg5 flox Primer B 5′-GTA CTG CAT AAT GGT TTA ACT CTT GC-3′ Sigma Oligo N/A
Atg5 flox Primer C 5′-ACA ACG TCG AGC ACA GCT GCG CAA GG-3′ Sigma Oligo N/A

Software and Algorithms

Graphpad Prism v. 7 www.graphpad.com N/A
FlowJo v. 10 www.flowjo.com N/A
R version 3.3.3 https://www.r-project.org/ N/A
Fiji https://fiji.sc N/A