REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
PE anti-mouse CD34 Antibody, Clone HM34 | BioLegend | Cat#152204; RRID: AB_313659 |
PE/Cy7 anti-mouse Ly-6G Antibody, Clone 1A8 | BioLegend | Cat#127618; RRID: AB_1877261 |
APC/Cy7 anti-mouse CD117 (c-kit) Antibody, Clone 2B8 | BioLegend | Cat#105826; RRID: AB_2632809 |
APC anti-mouse TER-119/Erythroid Cells Antibody, Clone Ter119 | BioLegend | Cat#116212; RRID: AB_313713 |
PE anti-mouse CD11b Antibody, Clone M1/70 | BioLegend | Cat#101208; RRID: AB_312790 |
APC anti-mouse/human CD45R/B220 Antibody | BioLegend | Cat#103212; RRID: AB_312996 |
APC anti-mouse CD8a Antibody, Clone 53-6.7 | BioLegend | Cat#100712; RRID: AB_312751 |
APC anti-mouse CD19 Antibody, Clone 6D5 | BioLegend | Cat#115511; RRID: AB_313646 |
Anti-mouse monoclonal G-CSF R/CD114 Antibody (723806); Alexa Fluor-405 | R&D Systems | Cat#MAB6039; RRID: AB_10890232 |
APC anti-mouse CD4 Antibody, Clone GK1.5 | BioLegend | Cat#100412; RRID: AB_312696 |
Alexa Fluor 488 anti-mouse CD36 Antibody, Clone G8.8 | BioLegend | Cat#102607; RRID: AB_1134107 |
Bacterial and Virus Strains | ||
Staphylococcus aureus | ATCC | ATCC 29213 |
Chemicals, Peptides, and Recombinant Proteins | ||
May Grünwald Wright Giemsa stain | Thermo Scientific | Cat#69027 |
Glut1.RBD reagent GFP labeled | Metaflora | Cat#Glut1-G25 |
Recombinant murine IL-3 | Peprotech | Cat#213-13-100 |
Recombinant murine G-CSF | Peprotech | Cat#250-05-100 |
Lysostaphin from Staphylococcus | Sigma Aldrich | Cat#L7386 |
Etomoxir sodium salt hydrate | Sigma Aldrich | Cat#E1905 |
Diethyl-umbelliferyl phosphate | Sigma Aldrich | Cat#D7692 |
Orlistat | Sigma Aldrich | Cat#O4139 |
TaqMan Gene Expression Master Mix | Life Technologies | Cat#4369016 |
Critical Commercial Assays | ||
ATP BIOLUMINESCENCE ASSAY KIT HS II | Sigma Aldrich | Cat# 11699709001 |
Anti-Ly-6G MicroBead Kit, mouse | Miltenyi Biotec | Cat#130-092-332 |
SuperScript III Platinum One-Step qRT-PCR Kit | Thermo Fisher | Cat#11732020 |
Glycolysis Cell-Based Assay Kit | Cambridge Bioscience | Cat#600450 |
Seahorse CF Cell Mito and Glycolysis Stress Test Kits | Agilent | Cat#103015 |
GLUCOSE (GO) Assay kit | Sigma Aldrich | Cat#GAGO20 |
Experimental Models: Cell Lines | ||
32Dcl3 Cell line | ATCC | ATCC; CRL-11346 RRID; CVCL_0119 |
Experimental Models: Organisms/Strains | ||
Mouse; Atg7 flox, Atg7tm1Tchi; B6.Cg-Atg7 < tm1Tchi > | Komatsu et al., 2005 | MGI: 3587769 |
Mouse; Atg5 flox, B6.129S-Atg5 < tm1Myok > | Riken | RBRC: 02975 |
Mouse; Mx1-cre; C.Cg-Tg(Mx1-cre)1Cgn/J | Jackson Laboratories | JAX: #005673 |
Mouse; Vav-cre; B6.Cg-Tg(Vav1-icre)A2Kio/J | Jackson Laboratories | JAX: #008610 |
Mouse: Cebpa-cre; Cebpatm1.1(cre)Touw | Wölfler et al., 2010 | MGI: 4867437 |
Oligonucleotides | ||
Mx1Cre 1 5′ -CAT GTG TCT TGG TGG GCT GAG-3′ | Sigma Oligo | N/A |
Mx1Cre 2 5′ -CGC ATA ACC AGT GAA ACA GCA T-3′ | Sigma Oligo | N/A |
CebpaCre FW: 5′ -CGA TGC AAC GAG TGA TGA GGT TC-3′ | Sigma Oligo | N/A |
CebpaCre RV: 5′ -GCA CGT TCA CCG GCA TCA AC-3′ | Sigma Oligo | N/A |
Atg7 WT PCR 5′ -CCA TGC TGA TGG CTA ATG TCT C- 3′ | Sigma Oligo | N/A |
Atg7 flox FW 5′ -CTG CAG GAA TTC GAT ATC ATA ACT TCG- 3′ | Sigma Oligo | N/A |
Atg7 flox RV 5′ -GTC CAG AGT CCG GTC TCT GGT TG- 3′ | Sigma Oligo | N/A |
Atg5 flox Primer A 5′-GAA TAT GAA GGC ACA CCC CTG AAA TG-3′ | Sigma Oligo | N/A |
Atg5 flox Primer B 5′-GTA CTG CAT AAT GGT TTA ACT CTT GC-3′ | Sigma Oligo | N/A |
Atg5 flox Primer C 5′-ACA ACG TCG AGC ACA GCT GCG CAA GG-3′ | Sigma Oligo | N/A |
Software and Algorithms | ||
Graphpad Prism v. 7 | www.graphpad.com | N/A |
FlowJo v. 10 | www.flowjo.com | N/A |
R version 3.3.3 | https://www.r-project.org/ | N/A |
Fiji | https://fiji.sc | N/A |