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. 2017 Sep 22;7:12212. doi: 10.1038/s41598-017-12566-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of relative mRNA expression and melatonin levels in transgenic switchgrass leaves and their correlation analysis. (a,b) Relative expression of oAANAT (normalized to line5) and oHIOMT (normalized to line3) was detected by qRT-PCR; (c,d) Melatonin levels in different OE-oAANAT and OE-oHIOMT lines; (e,f) Correlation analysis of melatonin levels and relative expression of oAANAT and oHIOMT, respectively. The data represents the mean ± S.E. of triplicate experiments. * and ** indicate a significant difference from that of WT at P < 0.05 and P < 0.01, respectively.