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. 2017 Sep 22;7:12143. doi: 10.1038/s41598-017-12331-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Enhanced adaptive immunity to 5 Burkholderia pseudomallei antigens in survivors of melioidosis, 12 weeks after acute illness, compared to healthy seronegative controls. IFNγ ELISpot assays for HIA-Bp or one of 15 Bp proteins were performed on cryopreserved PBMC from 16 seronegative controls (black squares) and 30 acute melioidosis survivors (black triangles). PBMC samples from melioidosis survivors were taken 12 weeks after acute illness. Data shown for each protein antigen is the number of spot forming cells (SFC) per 10⁶ PBMC following subtraction of the number of SFC from culture with media alone. Error bars represent mean + SEM and statistical significance between groups was determined using the Mann Whitney test.