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. 2017 Sep 22;7:12143. doi: 10.1038/s41598-017-12331-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Magnitude of T cell responses to Bp antigens in survivors and fatal cases compared to T cell immunity to Bp antigen BPSL2096 is concentrated on 2 peptide epitopes. IFNγ T cell responses to all 15 Bp proteins and to a control T cell antigen pool were summed for each individual in the seronegative control (black squares, n = 18) melioidosis survivor (black triangles, n = 30) and melioidosis fatality (black circles, n = 12) groups. Data are shown for both acute melioidosis (A) and at 12 weeks post acute illness (B). Error bars represent mean + SEM and statistical significance between groups was determined using the Mann Whitney test. IFNγ ELISpot assays were performed for a BPSL2096 20mer peptide panel. A positive response (*) was defined as 2 SD above the mean of the media only control and is represented by a dotted line. Error bars represent mean + SEM (C).