Figure 5.

Perturbation Experiments Reveal the Molecular Basis of Termination and Branching

(A) Comparison between a representative reconstructed fourth mammary gland (right) in the presence of TGF-β1 soaked beads (blue spheres) and a simulated theoretical counterpart (left). This confirms tip termination in proximity (light blue asterisks) to TGF-β1 soaked beads.

(B) Comparison between a representative reconstructed fourth mammary gland (right) in the presence of control inactive beads soaked in PBS with 0.1% BSA (blue spheres) and a simulated theoretical counterpart (left).

(C) Comparison between a representative reconstructed third mammary gland (left) in the presence of FGF10 soaked beads (blue spheres) and a simulated theoretical counterpart with local 2-fold increase in branching rate (right). Active TEBs are marked by red arrowheads. Scale bar, 2 mm.

See also Figure S5.