Fig. 5.

Effect of bicalutamide on Testosterone-induced I-related metabolic pathways. The pre-treatment with bicalutamide (100 nM, Bic) prevented T-induced phosphorylation (p) of AKT, ERK1/2, mTOR and GSK3β (*P < 0.05, **P < 0.01 or ***P < 0.001 vs. T0), as depicted by the densitometric analysis, reported below each representative blot. Specific total proteins were used as loading controls. Results of densitometric analysis (mean ± SE) are expressed as ratio p-/total-protein, fold increase vs. T0, taken as 1; data derived from three/four experiments using different cell preparations