Fig. 4.

RFP peptides alter RAB25-driven cell proliferation. a Western blot analysis of mock vector and RAB25 overexpressing HEY, MDA-MB231, and MCF7 cells. b Western blot analysis for endogenous RAB11a in HEY, MDA231 and MCF7 isogenic lines with or without RAB25 overexpression. c Left panel compares the viability of untreated HEY cells under low-serum condition in mock or RAB25 expressing lines while right panel shows the effects of treatment with DMSO or RFP14, 26, and 32 (15 μM) on the viability of HEY mock or RAB25 expressing cell lines at 48 h post-treatment. d, e shows corresponding results from MCF7 isogenics and MDA-MB231 isogenics, respectively. RAB25 expression results in enhancement (HEY, MCF7) or inhibition (MDA-MB231) of cell proliferation, which is reversed by RFP14, but not RFP32. Images and data shown represent mean ± s.e.m. of triplicate biological replicates. *P < 0.05; **P < 0.01; ***P < 0.001, Student’s t-test. NS, not significant