Fig. 6.

RFP14 inhibits RAB25-dependent gene expression in ovarian cancer cells. a Relative RAB25 mRNA levels in pcDNA3-HEY cells treated with DMSO, RAB25-expressing HEY cells treated with DMSO, and RAB25-expressing HEY cells treated with RFP14 measured by RNA-Seq profiling. b, c Quantitative GSEA plots of RAB25_UP and RAB25_DOWN gene set enrichment within the RFP14 vs. DMSO gene expression profile in RAB25-expressing HEY cells. Both RAB25-depenent gene sets were significantly enriched in the RFP14 vs. DMSO profile as indicated by the GSEA results for RAB25_DOWN (ES = 0.70, NES = 1.35, P < 0.0001) and RAB25_UP (ES = −0.74, NES = −1.61, P < 0.0001). The data represents two technical replicates from two independent biological replicate experiments. ES, enrichment score; NES, normalized enrichment score; NS, not significant. ***P < 0.005, Student’s t-test