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. 2017 Sep 22;7:12207. doi: 10.1038/s41598-017-12367-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell migration under each treatment condition, graphically represented by the mean migration curves +/− standard deviation. Blue curve, control; black curve, pertuzumab 20 μg/mL; red curve, cetuximab 5 nM; green curve, cetuximab 5 nM + pertuzumab 20 μg/mL. Cell migration was measured by an IncuCyte Zoom scratch wound analyser for each cell line under the following treatment conditions: control; cetuximab 5 nM; pertuzumab 20 μg/mL; cetuximab 5 nM + pertuzumab 20 μg/mL; 10 Gy; 10 Gy + cetuximab 5 nM; 10 Gy + pertuzumab 20 μg/mL; 10 Gy + cetuximab 5 nM + pertuzumab 20 μg/mL. Each experiment was performed in triplicate.