Table 2.
Off target analysis of founder SGM animals. ITALIC UPPER CASE LETTERS are the PAM (NGG) sequences. Lower case letters are the 12 nucleotides adjacent to the PAM sequences. Underlined nucleotides indicate mismatches with the corresponding sgRNA sequence. #mm: number of mismatches. Ins del: insertion or deletion. “no” indicates no off target mutation identified.
| Gene/off-target # | Potential off target gene | SgRNA/off-target Sequence | #mm | Ins del |
|---|---|---|---|---|
| FOXN1 | GCAATGCTggggctgtgcggGGG | — | — | |
| OT-N-1 | NMNAT2 | GGCGGCTCggggctgtgcggAGG | 7 | no |
| OT-N-2 | ZNF831 | GGACGTGAggggctgtgcggAGG | 6 | no |
| OT-N-3 | WDR61 | TGAGCGTGggggctgtgcggAGG | 6 | no |
| OT-N-4 | ZMAT5 | TACCGCTCggggctgtgcggAGG | 8 | no |
| OT-N-5 | RAB11FIP4 | AGTGGGGAggggctgtgcggAGG | 7 | no |
| OT-N-6 | ENSOCUG00000014472 | GAGACGCGggggctgtgcggTGG | 4 | no |
| OT-N-7 | ACO2 | GAGTGCCTggggctgtgcggTGG | 4 | no |
| OT-N-8 | TMEM144 | ACAGAAGAggggctgtgcggTGG | 6 | no |
| OT-N-9 | PLXND1 | AGCTTCCCggggctgtgcggTGG | 6 | no |
| OT-N-10 | ARHGAP25 | GGAGGGCAggggctgtgcggTGG | 4 | no |
| IL2RG | GGAGCCACccgatccactggGGG | — | — | |
| OT-G-1 | DVL2 | GGCACAATccgatccactggAGG | 4 | no |
| OT-G-2 | RASGRF1 | GCCATCCAccgatccactggTGG | 6 | no |
| PRKDC | TTGTCGTGcggggaccgctgCGG | — | ||
| OT-S-1 | QARS | GAGACTGAcggggaccgctgGGG | 6 | no |
| OT-S-2 | DCAF1 | CCCAGAGAcggggaccgctgGGG | 8 | no |
| OT-S-3 | NAALAD2 | AGGGCCCCcggggaccgctgGGG | 6 | no |
| OT-S-4 | ENSOCUG00000010835 | TTCTGCCCcggggaccgctgTGG | 5 | no |
| RAG2 | GTGGCTGGgtaacggagaggAGG | — | — | |
| OT-R2-1 | ARHGEF2 | TGTTCTGAgtaacggagaggTGG | 5 | no |
| OT-R2-2 | APOBEC1 | AAGGCAGAgtaacggagaggGGG | 4 | no |