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. 2017 Sep 22;7:12218. doi: 10.1038/s41598-017-12484-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Optical clearing of the whole-organ using UbasM. (a) Procedure of the UbasM clearing protocol and hemisphere brain samples cleared with UbasM at 30 °C for 7 days, (b) hemisphere brain samples cleared with CUBIC at 30 °C for 7 days and (c) hemisphere brain samples cleared with ScaleS at 37 °C for 7 days. All hemisphere brain samples were from C57/BL 4-week-old mice. (d) Transmittance curves of mouse brain samples cleared with various clearing agents after 7 days as shown in (a-c). (e) Clearing performance of whole organs (from C57/BL 8-week-old female mouse) at 30 °C for 3~10 days (clearing time depends on tissue). Organs such as intestine, spleen and pancreas tend to become transparent more quickly. Scale bars, 5 mm.