Table 1.

Relative kinetic parameters of PfActI and α-actin.

	PfActI		α-actin
	Nucleated polymerization (NP)
	Initial	Stable	Initial	Stable
k+	1.56 ± 0.26*	0.50 ± 0.16	0.56 ± 0.16*	0.50 ± 0.10
Cc (µM)	0.00 ± 0.06	−0.02 ± 0.12	−0.04 ± 0.11	0.04 ± 0.07
k−	−0.01 ± 0.10	0.01 ± 0.06	0.02 ± 0.06	−0.02 ± 0.03
	Steady state (SS)
Cc (µM)	0.11 ± 0.03		0.06 ± 0.03
k− †	0.05 ± 0.02		0.03 ± 0.02

All values reported as mean ± standard deviation (PfActI: n = 5 in NP, n = 3 in SS, α-actin: n = 3 in both NP and SS).

*p = 0.01 (two-tailed Student’s t-test)

^†Relative k₋ calculated using steady state Cc and the relative elongation rate constant (k₊) from the stable phase of the nucleated polymerization assays.