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. 2017 Sep 22;7:12136. doi: 10.1038/s41598-017-12039-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vivo assay of apidaecin target proteins using the ARGO method in E. coli. The ASKA clone is a complete set of open reading frame clones of E. coli. Cells were cultivated in the presence of apidaecin at the minimal inhibitory concentration. Each plasmid-encoded candidate gene was expressed under control of IPTG-inducible lac promoter. The overexpressors, the gene products of which are directly or indirectly involved in the action of apidaecin, may exhibit reduced sensitivity against apidaecin depending on IPTG induction. ARGO assay was used to evaluate each overexpressor for sensitivity against apidaecin. The genes involved in translation were tested.