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. 2017 Sep 22;7:12136. doi: 10.1038/s41598-017-12039-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vivo inhibition of lacZ expression with different stop codons. (a) The strains used in this assay. Genome-encoded lacZ gene was modified to possess three stop codons, which were recognized by PrfA and/or PrfB. (b) SDS-PAGE and (c) immunoblot using anti-LacZ. Lane 1: Size marker, lanes 2, 3 and 4: MG1655(lacZ-UAA), 5, 6 and 7: MG1655(lacZ-UGA), and 8, 9 and 10: MG1655(lacZ-UAG). Lanes 3, 6, and 9: 250 μM apidaecin was added. Lanes 4, 7 and 10: 500 μM apidaecin was added. The amount of protein was normalized to the OD of the cells.