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. 2017 Sep 22;7:12196. doi: 10.1038/s41598-017-12404-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative expression of (A,D) von Willebrand factor, green fluorescence; (B,E) claudin-5, red fluorescence; (C,F) vessel-specific claudin-5, yellow overlay in midbrain microvessels (<100 µm in diameter) of (aged) 19 mo control (A,B,C) or nano-aerosol-exposed (D,E,F) rats at 28 days after the end of exposure. Graph shows quantification of fluorescence shown in panels C and F. A minimum of 3 locations on each section (2 sections per slide), 3 slides and n = 3 per group were analyzed. Scale bar = 100 µm. *p < 0.050 compared to control rat microvessels.