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. 2017 Sep 22;7:12196. doi: 10.1038/s41598-017-12404-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Synaptophysin expression (red fluorescence) from the midbrain region of (A) 12–13 wk control; (B) 12–13 wk nano-aerosol-exposed; (C) 19 mo control; and (D) 19 mo nano-aerosol-exposed rats at 28 days after the end of exposure. A minimum of 3 locations on each section (2 sections per slide), 2 slides and n = 3 per group were analyzed for total fluorescence. Relative fluorescence per unit area is represented in the graph shown. *P < 0.050 compared to 12–13 wk control; ^†p < 0.050 compared to 19 mo control. Scale bar = 1000 µm.