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. Author manuscript; available in PMC: 2017 Sep 23.
Published in final edited form as: J Mol Endocrinol. 2014 Jan 30;52(2):77–85. doi: 10.1530/JME-13-0136

Figure 1.

Figure 1

Upregulation of Igf2 expression during in vitro calcification of murine aortic VSMCs. (A) Calcium content was determined by quantification of HCL leaching (μg/mg protein) (n=3). (B) Fold change in Igf2 mRNA and (C) IGF2 protein expression (blot representative of three independent experiments) in VSMCs cultured with control [C] or 3 mM Pi [P] medium (n=4). (D) Fold change in mRNA expression of the osteogenic marker Runx2. VSMCs were treated with control (white bar) or calcifying (3 mM Na2HPO4/NaH2PO4; filled bar) medium. Results are presented as mean±s.e.m. ***P<0.001.