Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 22;7:12168. doi: 10.1038/s41598-017-12488-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Host preconditioning by CTX does not lead to increased availability of IL-7 in mice. (A) The IL-7 levels in Rag2KO mice but not CTX-treated mice lead to downregulation of IL-7Rα in adoptively transferred naïve T cells. Following the timeline depicted in the schema, splenocytes from CD45.1 mice were transferred into untreated mice (WT), CTX-treated mice, and Rag2KO mice. 16 hours later, peripheral blood and spleen samples were collected and examined for expression of IL-7Rα in transferred CD4+ and CD8+ T cells by flow cytometry. Numbers in representative histograms indicate the values of mean florescence intensity (MFI) of IL-7Rα. (B) The dynamic changes in IL-7Rα expression in donor T cells are driven by antigen encounter in CTX-conditioned mice. As shown in the schema, A20HA tumor-bearing mice were treated with CTX followed by co-transfer of HA-specific and OVA-specific CD4+ T cells. Tail blood was collected at the indicated time points and examined for IL-7Rα expression by FACS. IL-7Rα expression profiles relative to cell division in HA-specific and OVA-specific CD4+ T cells are shown in representative dot plots. Results from two independent experiments are summarized in graph at right.