FIG. 3.

Schematic representation of the 2D system used for cytotoxicity studies. A monolayer of CHs was formed on the bottom of the well and then incubated with polymer suspensions in growth media (a). CH (b) or MSC (c) viability was assessed by Alamar Blue assay after 1 day for PNVCL-L and PNVCL-H at different polymer concentrations (0.001–1 mg/mL). *^##**Indicate statistical significance (p < 0.05 and p < 0.01, respectively) versus corresponding hydrogel with concentration of 0.001 mg/mL. ^##Indicates statistical significance (p < 0.01) versus the cell viability of PNVCL-L with the same polymer concentration. CH, chondrocyte; MSC, mesenchymal stem cell. Color images available online at www.liebertpub.com/tea