FIG. 4.

Schematic representation of the 3D system for cell encapsulation, injection in a polydimethylsiloxane mold, and incubation at 37°C (a). CH viability on the day of preparation (day 0) and after 3 days (day 3) of encapsulation in PNVCL-L (20% w/v) and PNVCL-H (10% w/v) injected with three needles of size (18G, 21G, and 27G) (b). Live/dead imaging (live cells-green; dead cells-red) of CH-laden (c) and MSC-laden (d) hydrogels injected with a needle of 21G, at days 0 and 3 (scale bars, 100 μm). **Indicates significance (p < 0.01) between groups 21G and 27G for PNVCL-L. ^##Indicates significance (p < 0.01) versus the cell viability of PNVCL-H at day 0 for each gauge size. 3D, three-dimensional. Color images available online at www.liebertpub.com/tea